Comparative analysis of cellulose acetate hemoglobin electrophoresis and high performance liquid chromatography for quantitative determination of hemoglobin A2
Blood Research
; : 46-50, 2015.
Article
de En
| WPRIM
| ID: wpr-104393
Bibliothèque responsable:
WPRO
ABSTRACT
BACKGROUND: The present study is designed to evaluate the reliability and cost effectiveness of cellulose acetate Hb electrophoresis and high performance liquid chromatography (HPLC) in the determination of HbA2 levels. METHODS: The test population comprised 160 individuals divided into four groups: normal individuals, beta-thalassemia trait (BTT) patients, iron deficiency anemia (IDA) patients, and co-morbid patients (BTT with IDA). HbA2 levels determined using cellulose acetate Hb electrophoresis and HPLC were compared. RESULTS: HbA2 levels were found to be diagnostic for classical BTT using either method. In co-morbid cases, both techniques failed to diagnose all cases of BTT. The sensitivity, specificity, and Youden's index for detection of the co-morbid condition was 69% and 66% for HPLC and cellulose acetate Hb electrophoresis, respectively. CONCLUSION: This study revealed that semi-automated cellulose acetate Hb electrophoresis is more suitable for use in beta-thalassemia prevention programs in low-income countries like Pakistan. This technique is easily available, simple and cost effective.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pakistan
/
Hémoglobine A2
/
Cellulose
/
Chromatographie en phase liquide à haute performance
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Chromatographie en phase liquide
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Sensibilité et spécificité
/
Analyse coût-bénéfice
/
Bêta-Thalassémie
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Anémie par carence en fer
/
Électrophorèse
Type d'étude:
Diagnostic_studies
Limites du sujet:
Humans
Pays comme sujet:
Asia
langue:
En
Texte intégral:
Blood Research
Année:
2015
Type:
Article