Detection of Listeria monocytogenes in CSF from Three Patients with Meningoencephalitis by Next-Generation Sequencing
Journal of Clinical Neurology
;
: 446-451, 2016.
Article
Dans Anglais
| WPRIM
| ID: wpr-104822
ABSTRACT
BACKGROUND AND PURPOSE:
Encephalitis caused by Listeria monocytogenes (L. monocytogenes) is rare but sometimes fatal. Early diagnosis is difficult using routine cerebrospinal fluid (CSF) tests, while next-generation sequencing (NGS) is increasingly being used for the detection and characterization of pathogens.METHODS:
This study set up and applied unbiased NGS to detect L. monocytogenes in CSF collected from three cases of clinically suspected listeria meningoencephalitis.RESULTS:
Three cases of patients with acute/subacute meningoencephalitis are reported. Magnetic resonance imaging and blood cultures led to a suspected diagnosis of L. monocytogenes, while the CSF cultures were negative. Unbiased NGS of CSF identified and sequenced reads corresponding to L. monocytogenes in all three cases.CONCLUSIONS:
This is the first report highlighting the feasibility of applying NGS of CSF as a diagnostic method for central nervous system (CNS) L. monocytogenes infection. Routine application of this technology in clinical microbiology will significantly improve diagnostic methods for CNS infectious diseases.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Imagerie par résonance magnétique
/
Système nerveux central
/
Liquide cérébrospinal
/
Maladies transmissibles
/
Diagnostic précoce
/
Diagnostic
/
Encéphalite
/
Listeria
/
Listeria monocytogenes
/
Méningite à Listeria
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Journal of Clinical Neurology
Année:
2016
Type:
Article
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