Expression of Cyclooxygenase-2 in Human Intestinal Epithelial Cells Stimulated with Bacteroides fragilis Enterotoxin
Journal of Bacteriology and Virology
;
: 147-157, 2002.
Article
Dans Coréen
| WPRIM
| ID: wpr-105399
ABSTRACT
A ~20 kDa heat-labile toxin (BFT) produced by enterotoxigenic B. fragilis induces chemokine responses that may be associated with mucosal inflammation. To determine whether epithelial cells can contribute to BFT-induced inflammation, we assessed the expression of cyclooxygenase (COX)-2 in BFT-stimulated human intestinal epithelial cells. Human intestinal epithelial cell lines, HT-29 and Caco-2, were incubated with purified BFT. COX-2 mRNA and protein expression were measured by quantitative RT-PCR and Western blot analysis, respectively. BFT increased expression of COX-2, not that of COX-1, in human intestinal epithelial cell lines. Up-regulated COX-2 expression was paralleled by increased prostaglandin E2 (PGE2) production measured by the radioimmunoassay. PGE2 was predominantly secreted from the basolateral surface of BFT-treated epithelial cells, whereas lactate dehydrogenase was released predominantly from the apical surface. Moreover, the COX-2 expression and PGE2 production were significantly suppressed when NF-kappaB activity was inhibited. The basolateral secretion of PGE2 by up-regulated COX-2 in the BFT-stimulated colon epithelial cells seems to contribute to the inflammatory response in the underlying intestinal mucosa.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Bacteroides
/
Bacteroides fragilis
/
ARN messager
/
Dosage radioimmunologique
/
Dinoprostone
/
Technique de Western
/
Facteur de transcription NF-kappa B
/
Prostaglandin-endoperoxide synthases
/
Côlon
/
Entérotoxines
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
Journal of Bacteriology and Virology
Année:
2002
Type:
Article
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