Differential regulation of inducible nitric oxide synthase and cyclooxygenase-2 expression by superoxide dismutase in lipopolysaccharide stimulated RAW 264.7 cells
Experimental & Molecular Medicine
; : 629-637, 2009.
Article
de En
| WPRIM
| ID: wpr-10783
Bibliothèque responsable:
WPRO
ABSTRACT
Inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) have been known to be involved in various pathophysiological processes such as inflammation. This study was performed to determine the regulatory function of superoxide dismutase (SOD) on the LPS-induced expression of iNOS, and COX-2 in RAW 264.7 cells. When a cell-permeable SOD, Tat-SOD, was added to the culture medium of RAW 264.7 cells, it rapidly entered the cells in a dose-dependent manner. Treatment of RAW 264.7 cells with Tat-SOD led to decrease in LPS-induced ROS generation. Pretreatment with Tat-SOD significantly inhibited LPS-induced expression of iNOS and NO production but had no effect on the expression of COX-2 and PGE2 production in RAW 264.7 cells. Tat-SOD inhibited LPS-induced NF-kappaB DNA binding activity, IkappaBalpha degradation and activation of MAP kinases. These data suggest that SOD differentially regulate expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Superoxide dismutase
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Lignée cellulaire
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Régulation de l'expression des gènes
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Lipopolysaccharides
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Cytokines
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Facteur de transcription NF-kappa B
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Espèces réactives de l'oxygène
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Mitogen-Activated Protein Kinase Kinases
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Cyclooxygenase 2
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Nitric oxide synthase type II
Limites du sujet:
Animals
langue:
En
Texte intégral:
Experimental & Molecular Medicine
Année:
2009
Type:
Article