Histologic Characteristics and Mechanical Properties of Bovine Pericardium Treated with Decellularization and alpha-Galactosidase: A Comparative Study
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 368-379, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-109678
ABSTRACT
BACKGROUND:
Bioprostheses for cardiovascular surgery have limitations in their use following as calicification. alpha-galactosidase epitope is known as a stimulant of immune response and then shows a progressing calcification. The objective of this study was to evaluate histologic characteristics and mechanical properties of decellularization and treated with alpha-galactosidase. MATERIALS ANDMETHODS:
Bovine pericardial tissues were allocated into three groups fixation only with glutaraldehyde, decellularization with sodium dodesyl sulfate and decellularization plus treatment with alpha-galactosidase. We confirmed immunohistological characteristics and mechanical properties as fatigue test, permeability test, compliance test, tensile strength (strain) test and thermal stability test.RESULTS:
Decellularization and elimination of alpha-gal were confirmed through immunohistologic findings. Decellularization had decreased mechanical properties compared to fixation only group in permeability (before fatigue test p=0.02, after fatigue test p=0.034), compliance (after fatigue test p=0.041), and tensile strength test (p=0.00). The group of decellularization plus treatment with alpha-galactosidase had less desirable mechanical properties than the group of decellularization in concerns of permeability (before fatigue test p=0.043) and strain test (p=0.001).CONCLUSION:
Favorable decellularization and elimination of alpha-gal were obtained in this study through immunohistologic findings. However, those treatment including decellularization and elimination of alpha-gal implied the decreased mechanical properties in specific ways. We need more study to complete appropriate ioprosthesis with decellularization and elimination of alpha-gal including favorable mechanical properties too.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Péricarde
/
Perméabilité
/
Sodium
/
Entorses et foulures
/
Résistance à la traction
/
Bioprothèse
/
Glutaraldéhyde
/
Alpha-Galactosidase
/
Compliance
/
Fatigue
langue:
Anglais
Texte intégral:
The Korean Journal of Thoracic and Cardiovascular Surgery
Année:
2012
Type:
Article
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