Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples
Journal of Veterinary Science
;
: 253-256, 2017.
Article
Dans Anglais
| WPRIM
| ID: wpr-109772
ABSTRACT
There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex nested RT-PCR were 10(1.5) TCID₅₀/mL for type 2 PRRSV and 10² infected cells/mL for PCV2. The kappa statistic, which measures agreement between methods, was 0.867, indicating a good level of agreement. This RNA-based duplex RT-PCR approach can be another way to detect type 2 PRRSV and PCV2 simultaneously and with improved convenience.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
ARN
/
ARN messager
/
RNA-directed DNA polymerase
/
Circovirus
/
Virus du syndrome respiratoire et reproducteur porcin
/
Syndrome dysgénésique et respiratoire porcin
/
RT-PCR
/
Limite de détection
/
Méthodes
Type d'étude:
Etude diagnostique
langue:
Anglais
Texte intégral:
Journal of Veterinary Science
Année:
2017
Type:
Article
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