Development and evaluation of an immunochromatographic assay using a gp51 monoclonal antibody for the detection of antibodies against the bovine leukemia virus
Journal of Veterinary Science
;
: 479-487, 2016.
Article
Dans Anglais
| WPRIM
| ID: wpr-110496
ABSTRACT
Infection of cattle with bovine leukemia virus (BLV) has been observed and reported worldwide, including in Korea. The onsite identification of infected cattle would help decreasing and eradicating BLV infections on farms. Here, we present a new immunochromatographic assay that employs monoclonal antibodies (MAbs) for the detection of antibodies against BLV in the field. BLV envelope glycoprotein (gp)51 was expressed in E. coli, and MAbs against recombinant BLV gp51 were generated for the development of an immunochromatographic assay to detect BLV antibodies in cattle. The sensitivity and specificity of the assay were determined by comparing these results with those obtained from a standard enzyme linked immunosorbent assay (ELISA). A total of 160 bovine sera were used to evaluate the new immunochromatographic assay. Using ELISA as a reference standard, the relative specificity and sensitivity of this assay were determined to be 94.7% and 98%, respectively. Because of its high sensitivity and specificity, this BLV antibody detection assay would be suitable for the onsite identification of BLV infection in the field.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Test ELISA
/
Glycoprotéines
/
Anticorps antideltarétrovirus
/
Infections à deltarétrovirus
/
Chromatographie d'affinité
/
Sensibilité et spécificité
/
Leucose bovine enzootique
/
Virus de la leucémie bovine
/
Agriculture
/
Corée
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Journal of Veterinary Science
Année:
2016
Type:
Article
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