Effect of cultured chondrocyte-seeded chondroitin-sulfate conjugated type I collagen scaffold on cartilage regeneration
Journal of the Korean Society of Plastic and Reconstructive Surgeons
;
: 413-419, 2007.
Article
Dans Coréen
| WPRIM
| ID: wpr-113024
ABSTRACT
PURPOSE:
In this study, porous type I collagen scaffolds were cross-linked using dehydrothermal(DHT) treatment and/or 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide(EDC), in the presence and absence of chondroitin-6-sulfate(CS) and cultured autologous chondrocytes(Chondro) for cartilage regeneration.METHODS:
Cartilage defects were created in the proximal part of the ear of New Zealand rabbits. Four prepared types of scaffolds(n=4) were inserted. The groups included Chondro-Collagen-DHT(Group 1), Chondro- Collagen-DHT-EDC(Group 2), Chondro-CS-Collagen- DHT(Group 3), and Chondro-CS-Collagen-DHT-EDC (Group 4). Histomorphometric analysis and cartilage- specific gene expression of the reconstructed tissues were evaluated 4, 8, and 12 weeks after implantation .RESULTS:
EDC cross-linked groups 2 and 4 regenerated more cartilage than other groups. However, calcification was observed in the 4th week after implantation. CS did not increase chondrogenesis in all groups. Cartilage-specific type II collagen mRNA expression increased in the course of time in all groups.CONCLUSION:
EDC cross-linking methods maintain the scaffold and promote extracellular matrix production of chondrocytes.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Régénération
/
ARN messager
/
Cartilage
/
Expression des gènes
/
Chondroïtines sulfate
/
Chondrocytes
/
Chondrogenèse
/
Collagène de type I
/
Collagène de type II
/
Oreille
Limites du sujet:
Animaux
langue:
Coréen
Texte intégral:
Journal of the Korean Society of Plastic and Reconstructive Surgeons
Année:
2007
Type:
Article
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