Occurrence of a PCR-Positive but Culture-Negative Case for vanB Vancomycin-Resistant Enterococci in Stool Surveillance
Laboratory Medicine Online
;
: 264-268, 2013.
Article
Dans Coréen
| WPRIM
| ID: wpr-114466
ABSTRACT
We present here occurence of PCR-positive but culture-negative for vanB vancomycin-resistant enterococci (VRE) from an enrichment broth of a stool surveillance culture in a patient suffering from Parkinson's disease, who was transferred from a long-term care facility because of aspiration pneumonia. He developed VRE bacteriuria at the hospital day 42. vanA and vanB genes were detected from 6 microg/mL vancomycin-containing BBL Enterococcosel broth (BD), of which color changed to black after overnight incubation, by both Seeplex VRE detection (Seegene, Seoul, Korea) and Anyplex VanR real-time PCR (Seegene). Subculture of an aliquot of the blackened broth on blood agar plate produced only vanA VRE. All of the four subsequent consecutive surveillance cultures for 1 month until discharge at hospital day 75 resulted in PCR-positive but culture-negative for vanB VRE from the enrichment broths. Therefore, the presence of a non-enterococcal intrinsic reservoir bearing vanB is more likely than low burden of vanB VRE. Considering the rare occurrence of vanB VRE in Korea, vanB-positive PCR results from the enrichment broth requires confirmation by microbiological studies.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Maladie de Parkinson
/
Pneumopathie de déglutition
/
Stress psychologique
/
Bactériurie
/
Ursidae
/
Vancomycine
/
Réaction de polymérisation en chaîne
/
Enterococcus
/
Soins de longue durée
/
Agar-agar
Type d'étude:
Étude de dépistage
Limites du sujet:
Humains
Pays comme sujet:
Asie
langue:
Coréen
Texte intégral:
Laboratory Medicine Online
Année:
2013
Type:
Article
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