Transcriptome Analysis of the Effects of Gomisin A on the Recovery of Carbon Tetrachloride-Induced Damage in Rat Liver / 한국실험동물학회지
Laboratory Animal Research
; : 161-169, 2011.
Article
Dans En
| WPRIM
| ID: wpr-116714
Responsable en Bibliothèque :
WPRO
ABSTRACT
Gomisin A possesses a hepatic function-facilitating property in liver-injured rats. Its preventive action on carbon tetrachloride-induced cholestasis is due to maintenance of the function of the bile acids-independent fraction. To investigate alterations in gene expression after gomisin A treatment on injured rat liver, DNA microarray analyses were performed on a Rat 44K 4-Plex Gene Expression platform with duplicated reactions after gomisin A treatment. We identified 255 up-regulated and 230 down-regulated genes due to the effects of gomisin A on recovery of carbon tetrachloride-induced rat liver damage. For functional characterization of these genes, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes biochemical pathways analyses were performed. Many up-regulated or down-regulated genes were related to cell cycle or focal adhesion and cell death genes, respectively. Our microarray experiment indicated that the liver repair mechanism induced by gomisin A was strongly associated with increased gene expressions related to cell cycle and suppression of the gene expression related in cell death.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Bile
/
Carbone
/
Tétrachloro-méthane
/
Expression des gènes
/
Cycle cellulaire
/
Cholestase
/
Génome
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Mort cellulaire
/
Lignanes
/
Séquençage par oligonucléotides en batterie
Limites du sujet:
Animals
langue:
En
Texte intégral:
Laboratory Animal Research
Année:
2011
Type:
Article