Chimerism Proven by Flow Cytomety in the Setting of an ABO Discrepancy: A Case Report / 대한수혈학회지
Korean Journal of Blood Transfusion
;
: 247-252, 2009.
Article
Dans Coréen
| WPRIM
| ID: wpr-116846
ABSTRACT
Chimerism is an important, yet uncommon cause of ABO phenotype/genotype discrepancies. The propositus was a 28 year-old pregnant women who had an ABO discrepancy, expressing an A(weak)B RBC phenotype and AB reverse type. Sequencing of ABO exons 6 and 7 revealed a B101/O01 genotype. Neither analysis of 9 short tandem repeats (STR) loci nor HLA typing on DNA extracted from white blood cells demonstrated evidence of chimerism. However, flow cytometric analysis using a PE-conjugated anti-A mouse monoclonal antibody detected a small population (3.8%) of red cells expressing normal A antigen. Based on this, we suggest that flow cytometric analysis is an effective method for the identification of small chimeric populations of RBCs in the immunohematology laboratory.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phénotype
/
ADN
/
Test d'histocompatibilité
/
Chimère
/
Exons
/
Répétitions microsatellites
/
Femmes enceintes
/
Chimérisme
/
Cytométrie en flux
/
Génotype
Limites du sujet:
Animaux
/
Femelle
/
Humains
langue:
Coréen
Texte intégral:
Korean Journal of Blood Transfusion
Année:
2009
Type:
Article
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