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Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies
Journal of Korean Medical Science ; : 405-411, 2007.
Article Dans Anglais | WPRIM | ID: wpr-118036
ABSTRACT
The aim of this study was to ascertain the folate receptor (FR) targetability by an in vitro study and to acquire FR-targeted images in vivo models by using synthetic folate conjugates. PEG-folate was synthesized and labeled with (99m)Tc and fluorescein isothiocynate (FITC). Cell uptake studies were carried out in KB cells (FR-positive) and A549 cells (FR-negative) using FITC- and the (99m)Tc-labeled conjugates. The radiolabeled conjugate was intravenously injected to KB tumor xenografted mice. After it was injected, gamma images were recorded at 30 min, 1, 2, 3 and 4 hr. Cell uptake studies showed a difference between the KB cells and the A549 cells by flow cytometry analysis and gamma counting. On in vivo images, the tumor-tonormal muscle ratio was greater than 4. It ascertained that the PEG-folate conjugate specifically binds to the FR expressed on tumor cells in vitro. Moreover, it was possible to acquire the FR-targeted gamma images using PEG-folate conjugates in tumor models.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Polyéthylène glycols / Facteurs temps / Traitement d'image par ordinateur / Protéines de transport / Technétium / Fluorescéine-5-isothiocyanate / Récepteurs de surface cellulaire / Lignée cellulaire tumorale / Acide folique / Souris nude Type d'étude: Étude pronostique Limites du sujet: Animaux / Femelle / Humains langue: Anglais Texte intégral: Journal of Korean Medical Science Année: 2007 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Polyéthylène glycols / Facteurs temps / Traitement d'image par ordinateur / Protéines de transport / Technétium / Fluorescéine-5-isothiocyanate / Récepteurs de surface cellulaire / Lignée cellulaire tumorale / Acide folique / Souris nude Type d'étude: Étude pronostique Limites du sujet: Animaux / Femelle / Humains langue: Anglais Texte intégral: Journal of Korean Medical Science Année: 2007 Type: Article