Folate Receptor Targeted Imaging Using Poly (ethylene glycol)-folate: In Vitro and In Vivo Studies
Journal of Korean Medical Science
;
: 405-411, 2007.
Article
Dans Anglais
| WPRIM
| ID: wpr-118036
ABSTRACT
The aim of this study was to ascertain the folate receptor (FR) targetability by an in vitro study and to acquire FR-targeted images in vivo models by using synthetic folate conjugates. PEG-folate was synthesized and labeled with (99m)Tc and fluorescein isothiocynate (FITC). Cell uptake studies were carried out in KB cells (FR-positive) and A549 cells (FR-negative) using FITC- and the (99m)Tc-labeled conjugates. The radiolabeled conjugate was intravenously injected to KB tumor xenografted mice. After it was injected, gamma images were recorded at 30 min, 1, 2, 3 and 4 hr. Cell uptake studies showed a difference between the KB cells and the A549 cells by flow cytometry analysis and gamma counting. On in vivo images, the tumor-tonormal muscle ratio was greater than 4. It ascertained that the PEG-folate conjugate specifically binds to the FR expressed on tumor cells in vitro. Moreover, it was possible to acquire the FR-targeted gamma images using PEG-folate conjugates in tumor models.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Polyéthylène glycols
/
Facteurs temps
/
Traitement d'image par ordinateur
/
Protéines de transport
/
Technétium
/
Fluorescéine-5-isothiocyanate
/
Récepteurs de surface cellulaire
/
Lignée cellulaire tumorale
/
Acide folique
/
Souris nude
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Journal of Korean Medical Science
Année:
2007
Type:
Article
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