Involvement of Nuclear Factor Kappa B in High-Fat Diet-Related Pancreatic Fibrosis in Rats
Gut and Liver
;
: 381-387, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-119845
ABSTRACT
BACKGROUND/AIMS:
High-fat diets contribute to pancreatic fibrogenesis, but the pathogenesis remains unclear. This study investigated the role of nuclear factor kappa B (NF-kappaB) in high-fat diet-induced pancreatic fibrosis in rats.METHODS:
Male Wistar rats were fed a high-fat diet or standard normal chow for 20 weeks. Pancreatic fibrosis was determined by Sirius red staining. Immunohistochemical staining, reverse transcription-polymerase chain reaction and Western blotting were used to identify NF-kappaB-associated genes or protein expressions.RESULTS:
Inflammation, fat deposition, pancreatic stellate cell activation and fibrosis were observed in the pancreases of the high-fat diet group. NF-kappaB subunit p65 (NF-kappaB/p65) expression was localized to the nucleus, and intercellular adhesion molecule 1 (ICAM-1) was over-expressed. Pancreatic gene expression levels of NF-kappaB/p65, ICAM-1 and tumor necrosis factor alpha were all elevated significantly in rats fed a high-fat diet compared with control rats. Western blotting also revealed significantly increased levels of ICAM-1 and nuclear NF-kappaB/p65 in rats fed high-fat diets comparison with control rats.CONCLUSIONS:
NF-kappaB is involved in high-fat diet-related pancreatic fibrosis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pancréas
/
Fibrose
/
Expression des gènes
/
Technique de Western
/
Facteur de transcription NF-kappa B
/
Facteur de nécrose tumorale alpha
/
Rat Wistar
/
Molécule-1 d'adhérence intercellulaire
/
Cellules stellaires pancréatiques
/
Alimentation riche en graisse
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Gut and Liver
Année:
2012
Type:
Article
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