Development of Quantitative Real-Time PCR Primers for Detection of Streptococcus sobrinus
International Journal of Oral Biology
;
: 149-154, 2016.
Article
Dans Coréen
| WPRIM
| ID: wpr-124487
ABSTRACT
The purpose of this study was to develop Streptococcus sobrinus-specific qPCR primers based on the nucleotide sequence of the RNA polymerase β-subunit gene (rpoB). The specificity of the primers was determined by conventional polymerase chain reaction (PCR) with 12 strains of S. sobrinus and 50 strains (50 species) of non-S. sobrinus bacteria. The sensitivity of the primers was determined by quantitative real-time PCR (qPCR) with serial dilutions of the purified genomic DNAs (40 ng to 4 fg) of S. sobrinus ATCC 33478(T). The specificity data showed that the S. sobrinus-specific qPCR primers (RTSsob-F4/RTSsob-R4) detected only the genomic DNAs of S. sobrinus strains with a detection limit of up to 4 fg of S. sobrinus genomic DNA. Our results suggest that the RTSsob-F4/RTSsob-R4 primers are useful in detecting S. sobrinus with high sensitivity and specificity for epidemiological studies of dental caries.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Streptococcus
/
Bactéries
/
DNA-directed RNA polymerases
/
ADN
/
Séquence nucléotidique
/
Études épidémiologiques
/
Réaction de polymérisation en chaîne
/
Sensibilité et spécificité
/
Streptococcus sobrinus
/
Caries dentaires
Type d'étude:
Etude diagnostique
/
Étude observationnelle
langue:
Coréen
Texte intégral:
International Journal of Oral Biology
Année:
2016
Type:
Article
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