Monocyte Chemoattractant Protein-1 Upregulates Fibronectin Secretion by Human Peritoneal Fibroblasts / 대한신장학회잡지
Korean Journal of Nephrology
;
: 259-265, 2002.
Article
Dans Coréen
| WPRIM
| ID: wpr-125454
ABSTRACT
BACKGROUND:
High glucose upregulates MCP-1 expression in rat glomerular mesangial cells and in human peritoneal mesothelial cells. However, the role of high glucose-induced MCP-1 on the development and progression of diabetic renal injury and peritoneal injury during peritoneal dialysis(PD) using high glucose PD solutions are not clear. Since MCP-1 was shown to upregulate transforming growth factor-beta1(TGF-beta1) and collagen expression in lung fibroblasts, the present study investigated the effects of MCP-1 on fibronectin secretion by mouse mesangial cells(MMC), human peritoneal mesothelial cells (HPMC), and human peritoneal fibroblasts(HPFB).METHODS:
Synchronized cells were stimulated by different concentrations of MCP-1(0.1-100 ng/mL) or TGF-beta1(0.1-10 ng/mL) for 48 hours. Fibronectin protein secreted into the media was analyzed by Western blot analysis.RESULTS:
MCP-1 up to 100 ng/mL did not affect fibronectin secretion by MMC. TGF-beta1 10 ng/mL, however, increased fibronectin secretion by MMC 2.8 fold that of control. MCP-1 up to 100 ng/mL did not affect fibronectin secretion by HPMC. But, TGF-beta1 0.1 ng/mL increased fibronectin secretion by HPMC 1.8 fold compared to control. On the other hand, MCP-1 increased fibronectin secretion by HPFB in a dose-dependent manner. MCP-1 at 1-10 ng/mL significantly increased fibronectin when compared to M199 control. 100 ng/mL MCP-1 further increased fibronectin secretion by HPFB compared to 0.1-10 ng/mL MCP-1.CONCLUSION:
These results suggest a possible role for MCP-1 in the development and progression of peritoneal fibrosis and support the view that in addition to recruiting inflammatory cells MCP-1 may play a role in tissue fibrosis in other organs.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Fibrose
/
Monocytes
/
Technique de Western
/
Collagène
/
Fibronectines
/
Chimiokine CCL2
/
Cellules mésangiales
/
Facteur de croissance transformant bêta-1
/
Fibrose péritonéale
/
Fibroblastes
Limites du sujet:
Animaux
/
Humains
langue:
Coréen
Texte intégral:
Korean Journal of Nephrology
Année:
2002
Type:
Article
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