Matrix Metalloproteinase-2 Expression of the Injured Anterior Cruciate Ligament in the Knee and Changes in Relation to Signal Transduction Pathways / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 64-71, 2008.
Article
Dans Coréen
| WPRIM
| ID: wpr-126981
ABSTRACT
PURPOSE:
This study was designed to pursue the basic relationship between MMP-2 and ligament injury via ex vivo stretching of the anterior cruciate ligament (ACL) and its changes by signal transduction pathway. MATERIALS ANDMETHODS:
After femur-ACL-tibia complex was harvested from rats, release of MMP-2 in stretch group and non-stretch group was checked using gelatin zymography. Firstly, authors investigated whether MMP-2 was released from the ligament or not and if so, how long it was released. In addition, the complexes were divided into two groups. In group I, 5 and 12N weights were used to stretch the complex for 10, 30, and 60-minute. In group II, after the ligament complexes were stretched by 15N for 30 minutes, various signal pathway inhibitors such as pertusis toxin, SP600125, PD98059, KT5720, curcumin, and Bay11-7082 were added to their supernatants. MMP-2 activity was evaluated.RESULTS:
MMP-2 was immediately released after ligament injury and its activity was increased in proportion to stretching duration and magnitude. As for the signal pathways, inhibition of JNK, ERK, AP-1, and NF-k beta pathways caused MMP-2 expression to be decreased.CONCLUSION:
It is considered that the release of MMP-2 plays an important role in remodeling process after ACL injury.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pyrroles
/
Sulfones
/
Poids et mesures
/
Flavonoïdes
/
Carbazoles
/
Transduction du signal
/
Ligament croisé antérieur
/
Facteur de transcription AP-1
/
Matrix metalloproteinase 2
/
Curcumine
Limites du sujet:
Animaux
langue:
Coréen
Texte intégral:
Journal of Korean Orthopaedic Research Society
Année:
2008
Type:
Article
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