Cyclooxgenase-2 Expression in Chronic Inflammatory Bowel Disease:Comparing of Immunohistochemical Stain to Real-time PCR
Intestinal Research
;
: 128-134, 2008.
Article
Dans Coréen
| WPRIM
| ID: wpr-128540
ABSTRACT
BACKGROUND/AIMS:
This study compared the results of cyclooxygenase-2 (COX-2) expression in inflammatory bowel disease and tuberculous colitis as evident by immunochemical staining and real time polymerase chain reaction (PCR).METHODS:
Patients with ulcerative colitis (n=18), Crohn's disease (n=7), tuberculous colitis (n=7) and 10 normal controls were included. Biopsied colonic mucosa was simultaneously used for immunohistochemical staining and real time PCR.RESULTS:
Patients with inflammatory bowel disease and tuberculous colitis showed high COX-2 expression by both methods compared to the normal controls. In Crohn's disease patients, the real time PCR value correlated well staining grade; this correlation was not evident in ulcerative colitis patients. In real time PCR, grossly normal colonic mucosa in ulcerative colitis also showed higher expression of COX-2 than normal mucosa.CONCLUSIONS:
Real time PCR value of COX-2 is more representative of inflammation state in inflammatory bowel disease than the value from immunohistochemical staining.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Immunohistochimie
/
Maladies inflammatoires intestinales
/
Rectocolite hémorragique
/
Maladie de Crohn
/
Réaction de polymérisation en chaîne
/
Colite
/
Côlon
/
Cyclooxygenase 2
/
Réaction de polymérisation en chaine en temps réel
/
Inflammation
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
Intestinal Research
Année:
2008
Type:
Article
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