Fractionation of DNases Specific to Haemonchus contortus Intestine by Phenyl Sepharose Column / 한국실험동물학회지
Laboratory Animal Research
; : 139-144, 2010.
Article
Dans En
| WPRIM
| ID: wpr-129611
Responsable en Bibliothèque :
WPRO
ABSTRACT
Multiple DNases were identified from Haemonchus contortus intestine based on previous studies. The DNases detected at 34, 36 and 38.5 kDa had diverse characteristics. Some of them had characteristics similar to those of mammalians and others had unusual characteristics. This study was carried out to fractionate worm intestinal DNases from other proteins using phenyl Sepharose chromatographic methods. All DNases detected from Haemonchus contortus intestine were fractionated in the flowthrough of phenyl Sepharose, indicating the worm DNases are hydrophilic. The DNases were enriched five-fold in the flowthrough fraction while additional steps are required for isolation of the worm DNases. Thus, fractionation with phenyl Sepharose could be used as a good initial step to enrich and separate DNases from other proteins.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Agarose
/
Protéines
/
Chromatographie
/
Désoxyribonucléases
/
Haemonchus
/
Intestins
Type d'étude:
Prognostic_studies
langue:
En
Texte intégral:
Laboratory Animal Research
Année:
2010
Type:
Article