Alterations of epinephrine-induced gluconeogenesis in aging
Experimental & Molecular Medicine
;
: 334-340, 2009.
Article
Dans Anglais
| WPRIM
| ID: wpr-136586
ABSTRACT
The effects of glucagon and epinephrine on gluconeogenesis in young (4 month) and old (24 month) Fisher 344 rat hepatocytes were compared. In contrast to glucagon, which had a similar effect on gluconeogenesis in both young and old cells, epinephrine caused a smaller increase in gluconeogenesis in old rat hepatocytes than in young hepatocytes. beta2 adrenergic receptor (beta2-AR) expression slightly decreased in aged rat liver, and there were differences between young and old hepatocytes in their patterns of G protein coupled receptor kinases, which are involved in the activation of beta2-AR receptor signal desensitization. The major isoform of the kinase changed from GRK2 to GRK3 and the expression of beta-arrestin, which is recruited by the phosphorylated beta2-AR for internalization and degradation, increased in aged rat liver. GRK3 overexpression also decreased the glucose output from young rat hepatocytes. We conclude that an age-associated reduction in epinephrine-induced gluconeogenesis occurs through the epinephrine receptor desensitizing system.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphorylation
/
Rats de lignée F344
/
Vieillissement
/
Glucagon
/
Épinéphrine
/
Récepteurs bêta-2 adrénergiques
/
Agonistes bêta-adrénergiques
/
Kinase-2 associée au récepteur couplé à une protéine G
/
Kinase-3 associée au récepteur couplé à une protéine G
/
Néoglucogenèse
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Experimental & Molecular Medicine
Année:
2009
Type:
Article
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