Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency
Korean Journal of Veterinary Research
; : 189-192, 2016.
Article
de En
| WPRIM
| ID: wpr-13821
Bibliothèque responsable:
WPRO
ABSTRACT
The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r² values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log₁₀ and HI titer of 5 log₂ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Bronchite
/
Test ELISA
/
Tests d'inhibition de l'hémagglutination
/
Tests de neutralisation
/
Virus de la bronchite infectieuse
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Efficacité du vaccin
/
Hémagglutination
langue:
En
Texte intégral:
Korean Journal of Veterinary Research
Année:
2016
Type:
Article