IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans
The Korean Journal of Parasitology
;
: 99-102, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-138588
ABSTRACT
Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Parasitologie
/
Immunoglobuline G
/
Immunoglobuline M
/
Test ELISA
/
Anticorps antiprotozoaires
/
Toxoplasmose
/
Technique d'immunofluorescence indirecte
/
Techniques de laboratoire clinique
/
Iran
/
Affinité des anticorps
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
The Korean Journal of Parasitology
Année:
2012
Type:
Article
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