Anti-apoptotic effect of fermented Citrus sunki peel extract on chemical hypoxia-induced neuronal injury / 한국영양학회지
Journal of Nutrition and Health
;
: 451-456, 2015.
Article
Dans Coréen
| WPRIM
| ID: wpr-14025
ABSTRACT
PURPOSE:
Neuronal apoptotic events induced by aging and hypoxic/ischemic conditions is an important risk factor in neurodegenerative diseases such as ischemia stroke and Alzheimer's disease. The peel of Citrus sunki Hort. ex Tanaka has long been used as a traditional medicine, based on multiple biological activities including anti-oxidant, anti-inflammation, and anti-obesity. In the current study, we examined the actions of fermented C. sunki peel extract against cobalt chloride (CoCl2)-mediated hypoxic death in human neuroblastoma SH-SY5Y cells.METHODS:
Cell viability was measured by trypan blue exclusion. Expression of apoptosis related proteins and release of cytochrome c were detected by western blot. Production of intracellular reactive oxygen species (ROS) and apoptotic morphology were examined using 2',7'-dichlorofluorescin diacetate (DCF-DA) and 4',6-diamidino-2-phenylindole (DAPI) staining.RESULTS:
Exposure to CoCl2, a well-known mimetic agent of hypoxic/ischemic condition, resulted in neuronal cell death via caspase-3 dependent pathway. Extract of fermented C. sunki peel significantly rescued the CoCl2-induced neuronal toxicity with the cell viability and appearance of apoptotic morphology. Cytoprotection with fermented C. sunki peel extract was associated with a decrease in activities of caspase-3 and cleavage of poly (ADP ribose) polymerase (PARP). In addition, increase in the intracellular ROS and release of cytochrome c from mitochondria to the cytosol were inhibited by treatment with extract of fermented C. sunki peel.CONCLUSION:
Based on these data, fermented C. sunki peel extract might have a protective effect against CoCl2-induced neuronal injury partly through generation of ROS and effectors involved in mitochondrial mediated apoptosis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Bleu de trypan
/
Vieillissement
/
Survie cellulaire
/
Technique de Western
/
Facteurs de risque
/
Citrus
/
Mort cellulaire
/
Espèces réactives de l'oxygène
/
Cobalt
/
Apoptose
Type d'étude:
Etude d'étiologie
/
Facteurs de risque
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
Journal of Nutrition and Health
Année:
2015
Type:
Article
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