A Melting Method for RNA Extraction from the Mucosal Membrane of the Mouse Middle Ear
Yonsei Medical Journal
;
: 497-502, 2015.
Article
Dans Anglais
| WPRIM
| ID: wpr-141614
ABSTRACT
PURPOSE:
There is much confusion surrounding the methods of RNA extraction from the middle ear mucosa of mice. In this study, we worked to develop a "melting method," which is faster, purer, and more reliable than other methods in common use. MATERIALS ANDMETHODS:
Thirty-two ears were used for this study. Light microscopy with hematoxylin-eosin staining of the bullae, scanning electron microscopy (SEM), spectrophotometer analysis, and reverse transcription polymerase chain reaction were performed before and after melting the half lateral bullae, which were detached from the temporal bone by using a lateral retroauricular approach.RESULTS:
Each resected half bulla contained a well distributed mucosal membrane. After a TRIzol melting duration of 10-30 minutes, only mucosal marker (MUC5AC) was expressed without bony marker (total osteocalcin). The same results were determined from SEM.CONCLUSION:
This melting method, compared with stripping and irrigation methods, is effective and offers an easier, more robust approach to extracting RNA from the middle ear mucosal membranes of mice.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Spectrophotométrie
/
ARN messager
/
Microscopie électronique à balayage
/
RT-PCR
/
Oreille moyenne
/
Mucine-5AC
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Yonsei Medical Journal
Année:
2015
Type:
Article
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