Characterization and antimicrobial efficacy of Portland cement impregnated with silver nanoparticles
The Journal of Advanced Prosthodontics
;
: 217-223, 2017.
Article
Dans Anglais
| WPRIM
| ID: wpr-141839
ABSTRACT
PURPOSE:
This study investigated the effects of silver nanoparticle (SN) loading into hydraulic calcium silicate-based Portland cement on its mechanical, antibacterial behavior and biocompatibility as a novel dental bone substitute. MATERIALS ANDMETHODS:
Chemically reduced colloidal SN were combined with Portland cement (PC) by the concentrations of 0 (control), 1.0, 3.0, and 5.0 wt%. The physico-mechanical properties of silver-Portland cement nanocomposites (SPNC) were investigated through X-ray diffraction (XRD), setting time, compressive strength, solubility, and silver ion elution. Antimicrobial properties of SPNC were tested by agar diffusion against Streptococcus mutans and Streptococcus sobrinus. Cytotoxic evaluation for human gingival fibroblast (HGF) was performed by MTS assay.RESULTS:
XRD certified that SN was successfully impregnated in PC. SPNC at above 3.0 wt% significantly reduced both initial and final setting times compared to control PC. No statistical differences of the compressive strength values were detected after SN loadings, and solubility rates of SPNC were below 3.0%, which are acceptable by ADA guidelines. Ag ion elutions from SPNC were confirmed with dose-dependence on the concentrations of SN added. SPNC of 5.0 wt% inhibited the growth of Streptococci, whereas no antimicrobial activity was shown in control PC. SPNC revealed no cytotoxic effects to HGF following ISO 10993 (cell viability > 70%).CONCLUSION:
Addition of SN promoted the antibacterial activity and favored the bio-mechanical properties of PC; thus, SPNC could be a candidate for the futuristic dental biomaterial. For clinical warrant, further studies including the inhibitory mechanism, in vivo and long-term researches are still required.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Argent
/
Solubilité
/
Streptococcus mutans
/
Diffraction des rayons X
/
Calcium
/
Streptococcus sobrinus
/
Colloïdes
/
Substituts osseux
/
Résistance à la compression
/
Agar-agar
Type d'étude:
Guide de pratique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
The Journal of Advanced Prosthodontics
Année:
2017
Type:
Article
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