Evaluation of a Colorimetric Broth Microdilution Method for Antimicrobial Susceptibility Testing Using 2,3,5-Triphenyltetrazolium Chloride / 대한임상미생물학회지
Korean Journal of Clinical Microbiology
;
: 49-53, 2007.
Article
Dans Anglais
| WPRIM
| ID: wpr-14276
ABSTRACT
BACKGROUND:
The broth microdilution susceptibility testing method is considered a standard for determining minimum inhibitory concentrations, and the addition of the redox indicator 2,3,5-triphenyltetrazolium chloride (TTC) to the broth microdilution method simplifies and increases its objectivity. The current study evaluated the usefulness of a TTC-modified broth microdilution method for antimicrobial susceptibility test of frequently encountered clinical isolates.METHODS:
The minimal inhibitory concentrations (MICs) of 10 antimicrobials for 111 clinical isolates of four bacterial species, Staphylococcus aureus, Escherichia coli, Enterobacter cloacae, and Acinetobacter baumannii, were investigated by a modification of the Clinical and Laboratory Standards Institute (CLSI)-recommended broth microdilution method with the addition of 2,3,5-triphenyltetrazolium chloride (TTC). The inhibitory effects of TTC against 192 strains of 22 bacterial species isolated from clinical specimens were also evaluated.RESULTS:
The number of colonies of all 192 strains of 22 bacterial species grown on TTC-containing Mueller-Hinton agar did not differ from those grown on Mueller-Hinton agar only. The MICs with TTC were within 2 dilutions of those obtained by the CLSI method in 569 (97.6%) of 583 organism-antimicrobial agent combinations.CONCLUSIONS:
The colorimetric MIC method using TTC may be a useful surrogate of antimicrobial susceptibility testing for most of the frequently isolated bacteria.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Oxydoréduction
/
Staphylococcus aureus
/
Bactéries
/
Tests de sensibilité microbienne
/
Enterobacter cloacae
/
Acinetobacter baumannii
/
Agar-agar
/
Escherichia coli
Type d'étude:
Guide de pratique
langue:
Anglais
Texte intégral:
Korean Journal of Clinical Microbiology
Année:
2007
Type:
Article
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