Diagnosis of canine brucellosis using recombinant ribosomal protein L7/L12 / 대한수의학회지
Korean Journal of Veterinary Research
;
: 25-31, 2012.
Article
Dans Coréen
| WPRIM
| ID: wpr-145340
ABSTRACT
Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Placenta
/
Protéines ribosomiques
/
Brucella
/
Brucellose
/
Test ELISA
/
Sensibilité et spécificité
/
Clones cellulaires
/
Vétérinaires
/
Foetus avorté
/
Brucella canis
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
Korean Journal of Veterinary Research
Année:
2012
Type:
Article
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