Different inhibition characteristics of intracellular transglutaminase activity by cystamine and cysteamine
Experimental & Molecular Medicine
;
: 576-581, 2004.
Article
Dans Anglais
| WPRIM
| ID: wpr-145921
ABSTRACT
The treatment of cystamine, a transglutaminase (TGase) inhibitor, has beneficial effects in several diseases including CAG-expansion disorders and cataract. We compared the inhibition characteristics of cystamine with those of cysteamine, a reduced form of cystamine expected to be present inside cells. Cystamine is a more potent inhibitor for TGase than cysteamine with different kinetics pattern in a non- reducing condition. By contrast, under reducing conditions, the inhibitory effect of cystamine was comparable with that of cysteamine. However, cystamine inhibited intracellular TGase activity more strongly than cysteamine despite of cytoplasmic reducing environment, suggesting that cystamine itself inhibits in situ TGase activity by forming mixed disulfides.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Étude comparative
/
Transglutaminases
/
Cystamine
/
Mercaptamine
/
Lignée cellulaire tumorale
/
Antienzymes
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Experimental & Molecular Medicine
Année:
2004
Type:
Article
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