Detection of Respiratory Viruses in Children by Multiplex Reverse Transcriptase PCR, Direct Immunofluorescence Assay, and Shell Vial Culture / 대한임상미생물학회지
Korean Journal of Clinical Microbiology
;
: 110-115, 2009.
Article
Dans Coréen
| WPRIM
| ID: wpr-146801
ABSTRACT
BACKGROUND:
Direct immunofluorescence assay (DFA) and shell vial culture (SVC) have been used to diagnose respiratory viral infections. Recently a multiplex reverse transcriptase PCR (mRT-PCR) for 12 respiratory viruses has been introduced. We evaluated the diagnostic usefulness of these methods.METHODS:
Among 275 nasopharyngeal aspirates (NPAs) received from pediatric patients during the 3-month period from May through July, 2007, 122 samples were selected so as to include diverse viruses and varying numbers of DFA-positive cells for mRT-PCR. Also, the results of the 85 NPAs that had been analyzed by both DFA and SVC were reviewed retrospectively.RESULTS:
Detection rates for the seven major respiratory viruses, respiratory syncytial virus (RSV), influenza virus A and B, parainfluenza virus 1, 2, and 3, and adenovirus by DFA vs mRT-PCR were 32.0% and 55.7%, and by DFA vs SVC were 32.9% and 40.0%. A number of adenovirus detected by DFA vs mRT-PCR were 12 and 22, and by DFA vs SVC were 6 and 18. A number of RSV detected were 3 and 6, and 13 and 8, respectively.CONCLUSIONS:
mRT-PCR detected the respiratory viruses at the highest rate, followed by SVC and DFA in a decreasing order. However, DFA and multiplex PCR were more sensitive than SVC for RSV, while SVC was more sensitive than the other methods for adenovirus.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Orthomyxoviridae
/
Virus respiratoires syncytiaux
/
Virus
/
Adenoviridae
/
Technique d'immunofluorescence
/
RNA-directed DNA polymerase
/
Infections à Paramyxoviridae
/
Technique d'immunofluorescence directe
/
RT-PCR
/
Réaction de polymérisation en chaine multiplex
Type d'étude:
Etude diagnostique
Limites du sujet:
Enfant
/
Humains
langue:
Coréen
Texte intégral:
Korean Journal of Clinical Microbiology
Année:
2009
Type:
Article
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