Monitoring antibody titers to recombinant Core-NS3 fusion polypeptide is useful for evaluating hepatitis C virus infection and responses to interferon-alpha therapy
Journal of Korean Medical Science
; : 165-170, 1999.
Article
de En
| WPRIM
| ID: wpr-149195
Bibliothèque responsable:
WPRO
ABSTRACT
To evaluate the clinical feasibility of the antibody titer against a chimeric polypeptide (named Core 518), in which a domain of Core and NS3 of hepatitis C virus (HCV) was fused, ELISA was performed in a total of 76 serum samples. Each serum was serially diluted using two-fold dilution method with distilled water into 10 concentrations. They were all positive for second generation anti-HCV assay (HCV EIA II; Abbott Laboratories). Genotyping RT-PCR, quantitative competitive RT-PCR, and RIBA (Lucky Confirm; LG Biotech) were also assayed. Anti-Core 518 antibody was detected in x 12800 or higher dilutions of sera from 35 of 43 chronic hepatitis C (81.4%) and nine of 16 hepatocellular carcinoma sera (56.3%), one of four cirrhosis (25%), 0 of four acute hepatitis C, and one of nine healthy isolated anti-HCV-positive subjects (p=0.0000). The anti-Core 518 antibody titers were well correlated with the presence of HCV RNA in serum (p=0.002). The anti-Core 518 antibody titers decreased significantly in nine of ten responders to IFN-alpha treatment. Monitoring anti-Core 518 titers may be helpful not only for differentiating the status of HCV infection among patients with various type C viral liver diseases, but also for predicting responses to IFN-alpha treatment.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protéines de fusion recombinantes
/
ARN viral
/
Immunotransfert
/
Protéines du core viral
/
Hépatite C
/
Protéines virales non structurales
/
Hepacivirus
/
Antigènes de l'hépatite C
/
Anticorps de l'hépatite C
/
Interféron alpha-2
Type d'étude:
Prognostic_studies
Limites du sujet:
Adult
/
Aged
/
Female
/
Humans
/
Male
langue:
En
Texte intégral:
Journal of Korean Medical Science
Année:
1999
Type:
Article