Glucocorticoid Mechanism of Inhibition of the Inflammatory Cells in Lumbar Intervertebral Disc Cells Stimulated by TNF-alpha Production of Nuclear Factor-kappaB / 대한정형외과연구학회지
Journal of Korean Orthopaedic Research Society
;
: 80-87, 2010.
Article
Dans Coréen
| WPRIM
| ID: wpr-149511
ABSTRACT
PURPOSE:
To analyze the action mechanism of NF-kappaB, IkappaB-alpha and effect of the Dexamethasone (DEXA) in mediating this inflammation, after stimulating cultured herniated intervertebral disc cells with TNF-alpha. MATERIALS ANDMETHODS:
After cultured human intervertebral disc cells passaged three times, they were divided into four groups A control group (A), DEXA treatment group (B), TNF-alpha treated group (C), TNF-alpha and DEXA were treated at the same time (D). IL-6 and IL-1beta gene expression were measured with semi-quantitative RT-PCR. Western blot analysis was performed to measure protein expression of IkappaB-alpha in the above groups for 10 minutes, 1 hour, 2 hours. In addition, in order to explain the mechanism of NF-kappaB nuclear binding for each group, the nuclear amount of NF-kappaB binding in the nucleus is measured by EMSA.RESULTS:
In RT-PCR, expression of IL-6 and IL-1beta was greatest in group C, followed by group D, group A. IkappaB-alpha expression of the group treated with DEXA was not detected in Western blot results within 10 minutes. However, if stimulated by TNF-alpha, the DEXA was not inhibited of IkappaB-alpha concentration. After 1 hour and 2 hours, IkappaB-alpha levels were expressed by cells autonomously (autoregulatory induction). EMSA results expression levels in nuclear protein was maintained in accordance with protein expression.CONCLUSIONS:
Our study shows that DEXA inhibits the production of mediators such as inflammatory IL-6 and IL-1beta, however, may not inhibit the transcription of NF-kappaB stimulated by TNF-alpha.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Dexaméthasone
/
Protéines nucléaires
/
Expression des gènes
/
Technique de Western
/
Facteur de transcription NF-kappa B
/
Interleukine-6
/
Facteur de nécrose tumorale alpha
/
Négociation
/
Protéines I-kappa B
/
Inflammation
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
Journal of Korean Orthopaedic Research Society
Année:
2010
Type:
Article
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