The Role of Activation of MAP Kinase in Human Intestinal Epithelial Cells Stimulated with Bacteroides fragilis Enterotoxin
Journal of Bacteriology and Virology
;
: 83-90, 2004.
Article
Dans Coréen
| WPRIM
| ID: wpr-14985
ABSTRACT
A ~20 kDa heat-labile toxin (BFT) produced by enterotoxigenic B. fragilis induces chemokine responses that are associated with mucosal inflammation. In the present study, we assessed whether the activation of mitogen-activated protein kinase (MAPK) affects the levels of IL-8 and MCP-1 produced by BFT stimulation in human epithelial HT-29 cells. Human intestinal epithelial HT-29 cell lines were incubated with purified BFT. MAPK and AP-1 in HT-29 cells were measured by Western blot and luciferase assay, respectively. The expression of chemokines such as IL-8 and MCP-1 were determined by quantitative RT-PCR, ELISA, and luciferase assay. BFT stimulation activated MAPK such as ERK1/2 and p38 in HT-29 cells. Treatment with MAPK inhibitors attenuated BFT-induced expression of IL-8 and MCP-1. Transfection with mutant genes for Ras or c-Jun did not only suppressed AP-1 reporter genes, but also inhibited BFT-induced expression of IL-8 and MCP-1 reporter genes. These results suggest that Ras and MAPK cascade may act as the upstream signaling for the activation of AP-1, which induce chemokine expression in BFT-stimulated intestinal epithelial cells.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphotransferases
/
Protein kinases
/
Bacteroides
/
Bacteroides fragilis
/
Test ELISA
/
Transfection
/
Technique de Western
/
Interleukine-8
/
Gènes rapporteurs
/
Facteur de transcription AP-1
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
Journal of Bacteriology and Virology
Année:
2004
Type:
Article
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