The Effect of GCSB-5 a New Herbal Medicine on Changes in Pain Behavior and Neuroglial Activation in a Rat Model of Lumbar Disc Herniation
Journal of Korean Neurosurgical Society
; : 98-105, 2016.
Article
de En
| WPRIM
| ID: wpr-150269
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE: Lumbar disc herniation can induce sciatica by mechanical compression and/or chemical irritation. The aim of this study was to compare the effects of GCSB-5 (Shinbaro(R)) and NSAIDs on pain-related behavior and on the expressions of microglia, astrocytes, CGRP, TRPV1, IL-6, and CX3CL1 in a rat model of lumbar disc herniation. METHODS: 112 male Sprague-Dawley rats underwent implantation of nucleus pulposus to a dorsal root ganglion (DRG). Rats were divided into five groups as follows; a saline group (the vehicle control group) (n=27), a 10 mg/kg aceclofenac group (the aceclofenac group) (n=22), and 100, 300 or 600 mg/kg GCSB-5 groups (the GCSB-5 100, 300, or 600 groups) (n=21 for each group). Rats were tested for mechanical allodynia at 3 days after surgery and at 1 day, 3 days, 7 days, 14 days, 21 days, 28 days, 35 days, 42 days, 49 days, and 56 days after treatment commencement. Immunohistochemical staining of microglia (Iba1), astrocytes (GFAP), CGRP, and TRPV1, and PCR for IL-6 and CX3CL1 were performed on spinal dorsal horns and DRGs at 56 days after medication commencement. RESULTS: After 56 days of GCSB-5 300 administration, mechanical withdrawal thresholds were significantly increased (p<0.05), and immunohisto-chemical expressions of Iba1, GFAP, CGRP, and TRPV1 were reduced than other groups, but this difference was not statistically significant. CONCLUSION: These results indicate GCSB-5 reduces mechanical allodynia and downregulates neuroglial activity and the expressions of CGRP and TRPV1 in the spinal segments of a rat model of lumbar disc herniation.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sciatalgie
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Peptide relié au gène de la calcitonine
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Anti-inflammatoires non stéroïdiens
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Astrocytes
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Réaction de polymérisation en chaîne
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Interleukine-6
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Groupes homogènes de malades
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Rat Sprague-Dawley
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Microglie
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Modèles animaux
Type d'étude:
Prognostic_studies
Limites du sujet:
Animals
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Humans
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Male
langue:
En
Texte intégral:
Journal of Korean Neurosurgical Society
Année:
2016
Type:
Article