Proteomic Approach for Identifying Marker Proteins in the Urinary Bladder Precancerous Conditions Induced by BBN(N-butyl-N-(4-hydroxybutyl) nitrosamine) / 대한비뇨기과학회지
Korean Journal of Urology
;
: 77-83, 2004.
Article
Dans Coréen
| WPRIM
| ID: wpr-151786
ABSTRACT
PURPOSE:
Simple and noninvasive methods for the diagnosis of transitional cell carcinoma of the bladder are needed for the prevention of invasive tumor. A proteomic technology has recently been developed to facilitate protein profiling of biological mixtures. We investigated the role of this proteomic approach as a possible tool to detect the marker protein during the initiation stages on BBN-induced bladder carcinogenesis in rats. MATERIALS ANDMETHODS:
Ten rats group A were given 0.05% BBN in drinking water for 12 weeks. Ten rats in group B were designated as a control group and were not given BBN. Whole urinary bladders of all rats were excised at 12 weeks from the beginning of the experiment. Conventional proteomics was performed with high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry.RESULTS:
A comparison of urinary bladder hyperplasia tissue with control tissue showed that five proteins; actin gamma2 propeptide, cytokeratin-20, proapolipoprotein, alpha2 actin(alpha-cardiac actin) and heat shock 27kDa protein 1 were over-expressed in hyperplastic tissues. Three protein; transcription factors, seminal vesicle secretory protein VI precursor and hypothetical protein RMT-7 were under-expressed in hyperplastic tissues.CONCLUSIONS:
In an animal model system, BBN-induced, urinary bladder mucosal hyperplasia resulted in an increase in five proteins and a decrease in three proteins. Of these altered proteins, CK-20 and SVS-VI seem to be important. The proteomic approach may be a simple and noninvasive method for monitoring and follow-up of bladder cancer patients. However more information is needed regarding CK-20 expression in nonmalignant urological disease and in human tumor tissue, and regarding SVS-VI expression in other organs, for clinical usage.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
États précancéreux
/
Vésicules séminales
/
Choc
/
Spectrométrie de masse
/
Facteurs de transcription
/
Maladies urologiques
/
Eau de boisson
/
Vessie urinaire
/
Tumeurs de la vessie urinaire
/
Carcinome transitionnel
Type d'étude:
Etude diagnostique
/
Étude observationnelle
/
Étude pronostique
Limites du sujet:
Animaux
/
Humains
langue:
Coréen
Texte intégral:
Korean Journal of Urology
Année:
2004
Type:
Article
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