Effect of Nitric Oxide on the Proliferation of Cultured Porcine Trabecular Meshwork Cells
Korean Journal of Ophthalmology
; : 1-6, 2003.
Article
de En
| WPRIM
| ID: wpr-152372
Bibliothèque responsable:
WPRO
ABSTRACT
To investigate the effect of nitric oxide (NO) on the proliferation of trabecular meshwork (TM) cells, primarily cultured porcine TM cells were exposed to NO donor (SNAP, -nitroso-N-acetyl-D, L-penicillamine) with and without its inhibitor (L-NAME, N (w) -Nitro-L-arginine methyl ester). The proliferation of TM cells was quantified by a rapid colorimetric assay. Acridine orange/Hoechest 33342 staining and flow cytometry with annexin-PI were done. As a result, NO inhibited the proliferation of TM cells significantly in a dose-dependent manner and this inhibitory effect was abolished by L-NAME. Fluorescent microscopy and flow cytometric analysis revealed that NO induced apoptotic cell death. The current results suggest that NO inhibit the proliferation of TM cells and apoptosis may be involved in some degree.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Suidae
/
Réseau trabéculaire de la sclère
/
Benzimidazoles
/
Orange acridine
/
Division cellulaire
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Survie cellulaire
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Cellules cultivées
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Donneur d'oxyde nitrique
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N-Acétyl-S-nitroso-pénicillamine
/
Cytométrie en flux
Limites du sujet:
Animals
langue:
En
Texte intégral:
Korean Journal of Ophthalmology
Année:
2003
Type:
Article