Pro-inflammatory cytokine expression in human gingival fibroblasts by Tannerella forsythia whole bacteria, membrane proteins, and lipopolysaccharide / 대한치주과학회지
The Journal of the Korean Academy of Periodontology
;
: 543-550, 2008.
Article
Dans Coréen
| WPRIM
| ID: wpr-152425
ABSTRACT
PURPOSE:
The purpose of this study was to investigate induction of cytokine expression in human gingival fibroblasts (HGFs) by whole cell and the components of T. forsythia. MATERIAL ANDMETHODS:
After HGFs were treated with lipopolysaccharide (LPS), membrane protein isolated from T. forsythia or culture media of T. forsythia, the induction of interleukin (IL)-1, IL-6 and IL-8 was examined with real-time PCR and ELISA. Their induction ability of cytokines was compared with whole bacteria.RESULT:
The expression of IL-6 and IL-8 was significantly induced in HGFs by whole bacteria and membrane protein. The expression of IL-1beta was induced by membrane protein of T. forsythia, not by whole bacteria. LPS and condition media of T. forsythia slightly activated HGFs.CONCLUSION:
The membrane protein of T. forsythia could be one of virulence factors.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Bactéries
/
Test ELISA
/
Cytokines
/
Interleukine-8
/
Interleukines
/
Interleukine-6
/
Milieux de culture
/
Forsythia
/
Facteurs de virulence
/
Réaction de polymérisation en chaine en temps réel
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
The Journal of the Korean Academy of Periodontology
Année:
2008
Type:
Article
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