Localization of Intracellular IVIonoclonal Antibody Specific for IVlycobacteria in Experimentally Induced Pulmonary Tuberculosis
Journal of the Korean Radiological Society
; : 267-271, 1994.
Article
de Ko
| WPRIM
| ID: wpr-153405
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: To determine whether intracellular localization of intravenously injected infection specific MoAb can be localized in the infected cells, immunohistochemical staining was obtained in animals infected with Mycobacterium tuberculosis. MATERIALS AND METHODS: One mg of intact mouse MoAb against mwobacteria (group I, n=10) and F(ab')2 (group II, n=6) were intravenously injected to the rabbits of each groups infected with M. tuberculosis H37RV. Immunohistochemical staining using an anti-mouse antibody was obtained at the 1,3, 5, 7 and 8th days in Group I and at the 1, 2, 3rd days in group II by streptavidin biotin method. For the control study, I mg of non specific polyclonal human IgG (group III, n=10) and 100 microgram of normal rabbit IgG F(ab')2 (group IV, n=6) were injected to the rabbits and guinea pigs respectively. RESULTS: Both groups (group I & II) showed a positive Ag-Ab reaction within the cytoplasm of monocytes. A weak but positive reaction was observed intracellularly in group III, however no positive reaction in group IV. CONCLUSION: Our results suggest intracellular Ag-Ab reaction plays an important role in the localization of infection by immunoscintigraphy using a specific MoAb fragments.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Tuberculose
/
Tuberculose pulmonaire
/
Biotine
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Immunoglobuline G
/
Monocytes
/
Streptavidine
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Cytoplasme
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Cochons d'Inde
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Mycobacterium tuberculosis
Limites du sujet:
Animals
/
Humans
langue:
Ko
Texte intégral:
Journal of the Korean Radiological Society
Année:
1994
Type:
Article