Expression and Evaluation of Chikungunya Virus E1 and E2 Envelope Proteins for Serodiagnosis of Chikungunya Virus Infection
Yonsei Medical Journal
;
: 828-835, 2008.
Article
Dans Anglais
| WPRIM
| ID: wpr-153693
ABSTRACT
PURPOSE:
Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIKV fever, which is a mosquitoe-transmitted viral disease in Africa, India, South-East Asia, and recently Southern Europe. Currently, serological diagnostic tests such as hemagglutination inhibition test (HI test), in-house IgM capture enzyme-linked immunosorbent assays (ELISA), and indirect immunofluorescence test were used for diagnosis of chikungunya fever, which are based on whole virus antigens. MATERIALS ANDMETHODS:
CHIKV E1, and E2 envelope proteins for the CHIKV-specific serodiagnostic reagents for chikungunya fever were expressed in baculovirus expression system. The seroreactivity of recombinant CHIKV E1 and E2 envelope proteins were evaluated using sera panels of patients from Laboratoire Marcel Merieux by indirect IgM capture ELISA.RESULTS:
The recombinant CHIKV E1 and E2 envelope protein showed sensitivity of 77.5% and 90%, respectively. The specificities of both CHIKV E1 and E2 envelope proteins were 100%.CONCLUSION:
The recombinant CHIKV E1 and E2 envelope proteins could be a useful diagnostic reagent for CHIKV infection.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protéines recombinantes
/
Test ELISA
/
Tests sérologiques
/
Cellules cultivées
/
Virus du chikungunya
/
Protéines de l'enveloppe virale
/
Baculoviridae
/
Sensibilité et spécificité
/
Clonage moléculaire
/
Infections à alphavirus
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Yonsei Medical Journal
Année:
2008
Type:
Article
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