Association of Transforming Growth Factor-beta 1 Gene Polymorphisms with Atherosclerotic Vascular Disease in Peritoneal Dialysis Patients / 대한신장학회지

ABSTRACT

PURPOSE: Atherosclerotic vascular disease (AVD) is a leading cause of morbidity and mortality in patients with end-stage renal disease (ESRD) on peritoneal dialysis (PD). Transforming growth factorbeta 1 (TGF-beta1) is a multifunctional cytokine that inhibits the atheromatous process. The author studied polymorphisms of the TGF-beta1 gene (-509C>T and 869T>C) as genetic susceptibility factors for AVD in PD patients. METHODS: Genotyping was carried out using the LightCycler 480 with melting curve analysis function. Prevalent vascular disease was defined by the presence of ischemic heart disease (IHD), peripheral vascular disease (PVD), cerebrovascular disease (CVD), or congestive heart failure (CHF). The presence of AVD was derived by the presence of any vascular disease (i.e., any degree of IHD/PVD/ CVD). RESULTS: In total, 109 PD patients were recruited (38.7% male, and 28.7% diabetic). The mean age was 49.1+/-14.1 years, and mean dialysis duration was 61.3+/-34.1 months. The most frequent genotype at -509C>T was CT (49.5%) and at 869T>C was TC (47.7%). No significant differences were observed in the genotype distributions of the investigated TGF-beta1 (CCCTTT, 18.5%55.6%25.9% vs 26.8%47.6%25.6%, Chi2=0.246, p=0.884; TTTCCC, 18.5%55.6%25.9% vs 28.0%45.1%26.8%, Chi2=1.188, p=0.552) between AVD group and no AVD group. CONCLUSION: TGF-beta1 gene polymorphisms at both -509C>T and +869T>C were not associated with an increased risk for prevalent vascular diseases. Further studies are required to evaluate the role of TGF-beta1 as a candidate gene.