The Diagnosis of Tuberculous Meningitis by Polymerase Chain Reaction
Journal of the Korean Neurological Association
;
: 8-13, 1992.
Article
Dans Coréen
| WPRIM
| ID: wpr-157082
ABSTRACT
Polymerase chain reaction (PCR) method is a proced.ure for in vitro enzymatic ampli fication of a specific segment of DNA. PCR method was used to detect any M. tuberculi DNA in the 16 cerebrospinal fluid specimens from 6 patients clinically diagnosed as tuberculous meningitis. We synthesized two oligonucleotide primers derived from the sequence of a gene that codes for the 65-kilodalton antigen of M. tuberculosis. The amplified 165bp genomic DNA of M. tuberculosis was detected in 3 specimens (18.5%) with polyacrylamide gel electrophoresis. A following southem blot analysis confirmed these mycobacterial DNAs and detected another amplified DNA (25%) that was not seen on the polyacrylamide gels. Conventional detection methods such as smear and culture for M. tuberculosis found these specimens to be negative. Now we recommend PCR and combined southem blot analysis as a useful tool for early and rapid diagnosis of tuberulous meningitis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Tuberculose
/
Méningite tuberculeuse
/
ADN
/
Liquide cérébrospinal
/
Réaction de polymérisation en chaîne
/
Gènes vif
/
Amorces ADN
/
Diagnostic
/
Électrophorèse sur gel de polyacrylamide
/
Gels
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
Journal of the Korean Neurological Association
Année:
1992
Type:
Article
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