Effect of Prunetin on TNF-alpha-Induced MUC5AC Mucin Gene Expression, Production, Degradation of IkappaB and Translocation of NF-kappaB p65 in Human Airway Epithelial Cells / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 205-209, 2013.
Article
Dans Anglais
| WPRIM
| ID: wpr-157866
ABSTRACT
BACKGROUND:
We investigated whether prunetin significantly affects tumor necrosis factor-alpha (TNF-alpha)-induced MUC5AC mucin gene expression, production, inhibitory kappa B (IkappaB) degradation and nuclear factor kappa B (NF-kappaB) p65 translocation in human airway epithelial cells.METHODS:
Confluent NCI-H292 cells were pretreated with prunetin for 30 minutes and then stimulated with TNF-alpha for 24 hours or the indicated periods. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The effect of prunetin on TNF-alpha-induced degradation of IkappaB and translocation of NF-kappaB p65 was investigated by western blot analysis.RESULTS:
We found that incubation of NCI-H292 cells with prunetin significantly inhibited mucin production and down-regulated the MUC5AC gene expression induced by TNF-alpha. Prunetin inhibited TNF-alpha-induced degradation of IkappaB and translocation of NF-kappaB p65.CONCLUSION:
This result suggests that prunetin inhibits the NF-kappaB signaling pathway, which may explain its role in the inhibition of MUC5AC mucin gene expression and production regulated by the NF-kappaB signaling pathway.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Test ELISA
/
Expression des gènes
/
Technique de Western
/
Réaction de polymérisation en chaîne
/
Facteur de transcription NF-kappa B
/
Facteur de nécrose tumorale alpha
/
Transcription inverse
/
Cellules épithéliales
/
Mucine-5AC
/
Isoflavones
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Tuberculosis and Respiratory Diseases
Année:
2013
Type:
Article
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