Your browser doesn't support javascript.
loading
Identification of Epstein-Barr Virus in the Human Placenta and Its Pathologic Characteristics
Journal of Korean Medical Science ; : 1959-1966, 2017.
Article Dans Anglais | WPRIM | ID: wpr-159414
ABSTRACT
Epstein-Barr virus (EBV), a common pathogen in humans, is suspected as the cause of multiple pregnancy-related pathologies including depression, preeclampsia, and stillbirth. Moreover, transmission of EBV through the placenta has been reported. However, the focus of EBV infection within the placenta has remained unknown to date. In this study, we proved the expression of latent EBV genes in the endometrial glandular epithelial cells of the placenta and investigated the cytological characteristics of these cells. Sixty-eight placentas were obtained from pregnant women. Tissue microarray was constructed. EBV latent genes including EBV-encoding RNA-1 (EBER1), Epstein-Barr virus nuclear antigen 1 (EBNA1), late membrane antigen (LMP1), and RPMS1 were detected with silver in situ hybridization and/or mRNA in situ hybridization. Nuclear features of EBV-positive cells in EBV-infected placenta were compared with those of EBV-negative cells via image analysis. Sixteen placentas (23.5%) showed positive expression of all 4 EBV latent genes; only the glandular epithelial cells of the decidua showed EBV gene expression. EBV infection status was not significantly correlated with maternal, fetal, or placental factors. The nuclei of EBV-positive cells were significantly larger, longer, and round-shaped than those of EBV-negative cells regardless of EBV-infection status of the placenta. For the first time, evidence of EBV gene expression has been shown in placental tissues. Furthermore, we have characterized its cytological features, allowing screening of EBV infection through microscopic examination.
Sujets)

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Placenta / Pré-éclampsie / Argent / ARN messager / Expression des gènes / Dépistage de masse / Hybridation in situ / Latence virale / Herpèsvirus humain de type 4 Type d'étude: Etude diagnostique / Étude pronostique / Étude de dépistage Limites du sujet: Femelle / Humains langue: Anglais Texte intégral: Journal of Korean Medical Science Année: 2017 Type: Article

Documents relatifs à ce sujet

MEDLINE

...
LILACS

LIS

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Placenta / Pré-éclampsie / Argent / ARN messager / Expression des gènes / Dépistage de masse / Hybridation in situ / Latence virale / Herpèsvirus humain de type 4 Type d'étude: Etude diagnostique / Étude pronostique / Étude de dépistage Limites du sujet: Femelle / Humains langue: Anglais Texte intégral: Journal of Korean Medical Science Année: 2017 Type: Article