Gene expression profiling of mouse aborted uterus induced by lipopolysac charide / 대한해부학회지
Anatomy & Cell Biology
;
: 98-105, 2011.
Article
Dans Anglais
| WPRIM
| ID: wpr-159931
ABSTRACT
To identify genes that participate in the abortion process, normal pregnant uteri were compared to lipopolysaccharide (LPS)-induced abortion uteri. At day 6 of pregnancy, mice were treated with LPS at various time points to induce an abortion. Total RNAs were applied to a cDNA microarray to analyze genes with altered expression. At the early stage (2 hours) of LPS-induced abortion, upregulated genes were mainly composed of immune responsive genes, including Ccl4, Ccl2, Cxcl13, Gbp3, Gbp2, Mx2, H2-Eb1, Irf1 and Ifi203. Genes related to toll-like receptor signaling were also overexpressed. At late stages of abortion (12-24 hours), many genes were suppressed rather than activated, and these were mainly related to the extracellular matrix, cytoskeleton, and anti-apoptosis. Altered expression of several selected genes was confirmed by real time reverse transcription-polymerase chain reaction. The results demonstrated that many known genes were altered in the LPS-treated pregnant uterus, implying that the molecular mechanisms of the genes involved in LPS-induced abortion are complicated. Further analysis of this expression profile will help our understanding of the pathophysiological basis for abortion.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Utérus
/
Cytosquelette
/
ARN
/
Expression des gènes
/
Séquençage par oligonucléotides en batterie
/
Analyse de profil d'expression de gènes
/
Récepteurs de type Toll
/
Matrice extracellulaire
Limites du sujet:
Animaux
/
Grossesse
langue:
Anglais
Texte intégral:
Anatomy & Cell Biology
Année:
2011
Type:
Article
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