Expression Profiles of F4/80 and Nestin in Ocular Immune Cells Following Pharmaceutically Induced Retinal Degeneration in Adult Mice
Journal of the Korean Ophthalmological Society
;
: 945-953, 2013.
Article
Dans Coréen
| WPRIM
| ID: wpr-160293
ABSTRACT
PURPOSE:
To evaluate the expression patterns of F4/80 and nestin in the ciliary body and the optic nerve following N-methyl-N-nitrosourea (NMU)-induced retinal degeneration in adult mice.METHODS:
After intraperitoneal injection of MNU (60 mg/kg) in adult mice, the eyes were enucleated at 2, 4, 7 and 30 days. Hematoxylin and eosin (H&E) stain, terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stain and immunohistochemical stains of F/80 and nestin were performed.RESULTS:
After MNU treatment, the photoreceptors were destroyed by cell apoptosis. According to immunohistochemistry, F4/80 and nestin were not co-expressed in the control group, but F4/80 was expressed within the ciliary body and optic nerve in the MNU-treated group; the expression of nestin also increased. In the outer nuclear layer, F4/80 and nestin co-expressing cells were observed.CONCLUSIONS:
In response to retinal damage, the F4/80 and nestin co-expressing cells migrated to the retina from the ciliary body and optic nerve and were activated.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Nerf optique
/
Rétine
/
Dégénérescence de la rétine
/
Rétinal
/
Immunohistochimie
/
Corps ciliaire
/
Apoptose
/
Éosine jaunâtre
/
Agents colorants
/
Oeil
Limites du sujet:
Animaux
/
Humains
langue:
Coréen
Texte intégral:
Journal of the Korean Ophthalmological Society
Année:
2013
Type:
Article
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