Polymerase Chain Reaction in the Diagnosis of Infectious Endophthalmitis
Journal of the Korean Ophthalmological Society
;
: 600-606, 2001.
Article
Dans Coréen
| WPRIM
| ID: wpr-168606
ABSTRACT
PURPOSE:
We evaluated the efficacy of the PCR which is known as more sensitive method than culture in the diagnosis of causal microorganisms of the infective endophthalmitis.METHODS:
We used 0.3 ml of aqueous humor and 0.5 ml of vitreous sample in 3 cases of postoperative and 1 case of endogenous endophthalmitis for detecting causal microorganisms. To detect the bacteria we used universal, Gram positive and negative primers, and to detect the fungus we used fungal primer.RESULTS:
Three cases of endophthalmitis, there was no bacteria in the bacterial culture for 10 days but PCR results identified causal microorganisms in all cases.CONCLUSIONS:
PCR is effective in the fast and accurate diagnosis of infective endophthalmitis and especially in the culture-negative endophthalmitis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Humeur aqueuse
/
Bactéries
/
Endophtalmie
/
Réaction de polymérisation en chaîne
/
Diagnostic
/
Champignons
Type d'étude:
Etude diagnostique
langue:
Coréen
Texte intégral:
Journal of the Korean Ophthalmological Society
Année:
2001
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS