Adenovirus Expressing Human Interferon Inhibits Replication of Foot and Mouth Disease Virus and Reduces Fatal Rate in Mice
Journal of Bacteriology and Virology
;
: 224-231, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-170983
ABSTRACT
Interferon is an important cytokine that plays a critical role in the initial host defense against viral infection. Recombinant human adenoviruses expressing human interferon-alpha (Ad-HIFNalpha) or pig interferon-beta fused with interleukin-18 (Ad-PIFNbeta-IL18) were constructed and used to induce an early protective response against foot and mouth disease (FMD). To analyze the antiviral effect, bovine thyroid and porcine kidney IBRS-2 cells and ICR mice were treated with Ad-HIFNalpha, Ad-PIFNbeta-IL18, and cocktail of Ad-HIFNalpha and Ad-PIFNbeta-IL18. The survival rate of suckling mice was monitored after foot and mouth disease virus (FMDV) challenge following intra-peritoneal (IP) administration of appropriate adenovirus. Indirect antigen ELISA was performed to evaluate inhibition of FMDV replication following challenge with the FMDV O, A, or Asia 1 serotypes in vitro. These recombinant adenoviruses reduced the replication of FMDV in susceptible cells, thereby decreasing the fatality in mice, suggesting that they can be a useful control method for the early protection against FMD infection in livestock after field trial.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Asie
/
Glande thyroide
/
Test ELISA
/
Adenoviridae
/
Adénovirus humains
/
Taux de survie
/
Interférons
/
Interféron bêta
/
Interféron alpha
/
Interleukine-18
Limites du sujet:
Animaux
/
Humains
Pays comme sujet:
Asie
langue:
Anglais
Texte intégral:
Journal of Bacteriology and Virology
Année:
2012
Type:
Article
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