Protective Effects of Epigallocatechin Gallate after UV Irradiation in Cultured Human Retinal Pigment Epithelial Cells
Korean Journal of Ophthalmology
; : 232-237, 2007.
Article
de En
| WPRIM
| ID: wpr-171843
Bibliothèque responsable:
WPRO
ABSTRACT
PURPOSE: To evaluate the protective effects of Epigallocatechin gallate (EGCG) against UV irradiation in cultured human retinal pigment epithelial (RPE) cells. METHODS: UV irradiation was produced by a UV lamp for 30 seconds with an irradiance of 3.3 mW/cm2. After 5 minutes and 1 hour, we administered different concentrations of EGCG (0, 5, 10, 15, 25, 50, 100 uM). The cell count was determined under a microscope using a counting chamber and the cell activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. RESULTS: The cell count of cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group, compared with the non-administrated group. The cell activity of the cultured human RPE cells after UV irradiation was markedly increased in the EGCG administration group and was increased in a dose-dependent way as determined by the MTT assay. CONCLUSIONS: The administration of EGCG increased the cell count and the cell activity after UV irradiation in cultured human retinal pigment epithelial cells; this suggests that EGCG provided protection against UV damage in cultured human retinal pigmented epithelial cells.
Mots clés
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Épithélium pigmentaire de l'oeil
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Lésions radiques
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Radioprotecteurs
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Spectrophotométrie
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Rayons ultraviolets
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Catéchine
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Numération cellulaire
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Cellules cultivées
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Relation dose-effet des rayonnements
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Antioxydants
Limites du sujet:
Humans
langue:
En
Texte intégral:
Korean Journal of Ophthalmology
Année:
2007
Type:
Article