Gender difference and change of alpha1-adrenoceptors in the distal mesenteric arteries of streptozotocin-induced diabetic rats / 대한마취과학회지
Korean Journal of Anesthesiology
;
: 419-427, 2011.
Article
Dans Anglais
| WPRIM
| ID: wpr-172267
ABSTRACT
BACKGROUND:
The purpose of this study was to evaluate the gender-related changes in the function and distribution of alpha1-adrenoceptors in the distal mesenteric artery of streptozotocin (STZ)-induced diabetic rats at the level of alpha1-adrenoceptor subtypes.METHODS:
Diabetes was induced by intravenous injection of STZ in a dose of 60 mg/kg through the tail vein in 8 week-old male or female Sprague-Dawley rats (n = 13/group). Age-matched normal rats (n = 15) were used as a control group. Four weeks after STZ injection, the change in mean arterial pressure caused by a 45degrees tilting was recorded. The alpha1-adrenoceptor subtypes mediating contractions of the distal mesenteric artery were investigated using the agonist, phenylephrine as well as subtype-selective antagonists including prazocin, 5-methylurapidil, and BMY 7378. The expression of alpha1-adrenoceptor subtypes of each artery was examined by immunofluorescence staining and western blotting using subtype selective antibodies.RESULTS:
Compared with normal male rats, the contractile response to phenylephrine was decreased in the distal mesenteric artery in normal female rats. Moreover, a decrease in contractile force was observed in STZ-induced diabetic rats compared with age-matched controls. Western blotting revealed that there was the difference between normal male and female rats in manifestation of the alpha1D-adrenoceptor. In STZ-induced male and female diabetic rats, all alpha1-adrenoceptor subtypes were decreased in distal mesenteric arteries, compared with normal rats.CONCLUSIONS:
There was the gender-related functional difference of alpha1-adrenoceptors in normal rats. In both male and female rats, diabetes decreased the contractile response in mesenteric arteries, which might be caused by the overall change in alpha1-adrenoceptor.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phényléphrine
/
Pipérazines
/
Artères
/
Veines
/
Technique de Western
/
Technique d'immunofluorescence
/
Négociation
/
Rat Sprague-Dawley
/
Streptozocine
/
Récepteurs alpha-1 adrénergiques
Limites du sujet:
Animaux
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Korean Journal of Anesthesiology
Année:
2011
Type:
Article
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