Efficacy of Purified Vi Polysaccharide Typhoid Vaccine
Journal of Veterinary Science
;
: 67-70, 2002.
Article
Dans Anglais
| WPRIM
| ID: wpr-172834
ABSTRACT
This experiment was conducted to assess the efficacy of typhoid vaccine newly produced by purifying Vi antigen of Salmonella typhi. With Karber method, LD50 of challenging organism (S. typhi ty2) was determined as 6.31 CFU/mouse, and then the organism was used for the study. With Probits method, ED50 of the vaccine was determined as 0.016 microgram / 0.5 ml / mouse. The ELISA titer (0.5097+/-0.0606) was 4 times in the group treated with high dose (0.25 microgram/0.5ml) as in control (0.1113+/-0.0110). Six major protein bands of 66, 55, 35, 33, 18, and 9 kd were detected in Western blot analysis with serum of a vaccine treated mouse, whereas only one weak band of about 35 kd was detected with serum of a control mouse. We concluded that typhoid vaccine produced by purifying Vi antigen of S. typhi very effectively prevent S. typhi infection in mice.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Polyosides bactériens
/
Salmonella typhi
/
Fièvre typhoïde
/
Test ELISA
/
Vaccins antityphoparatyphoïdiques
/
Modèles logistiques
/
Technique de Western
/
Dose létale 50
/
Souris de lignée BALB C
/
Anticorps antibactériens
Type d'étude:
Facteurs de risque
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Journal of Veterinary Science
Année:
2002
Type:
Article
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