Real-Time PCR analysis of af4 and dek genes expression in acute promyelocytic leukemiat (15;17)patients
Experimental & Molecular Medicine
;
: 279-282, 2004.
Article
Dans Anglais
| WPRIM
| ID: wpr-173474
ABSTRACT
Among several newly identified oncogenes, dek and af4 are attractive targets for researchers interested with leukemia. In this study quantitative Real-Time RT-PCR technique was used to define alterations in expression of dek and af4 genes associated with acute promyelocytic leukaemia (APL) t (15; 17). RNA samples obtained from bone marrow aspirates of fourteen APL patients, cDNA portions were labelled with Syber Green 1 dye and LightCycler analysis have been performed. Expression changes in patients were found not significant in comparison to healthy donors for af4 (P=0.192) and dek (P= 0.0895). We suggest that af4 gene may have a role in leukomogenesis restricted to lymphoblastic lineage; also further studies must carry on with a larger series of patients in order to understand the relationship between the dek gene and APL. Our study was the first attempt for analysing dek and af4 genes in APL t (15; 17) patients by quantitative Real-Time RT-PCR. This rapid and sensitive method could be used to screen these genes in different types of leukaemia.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Translocation génétique
/
Chromosomes humains de la paire 15
/
Chromosomes humains de la paire 17
/
ARN messager
/
Protéines nucléaires
/
Protéines chromosomiques nonhistones
/
Leucémie aiguë promyélocytaire
/
Régulation négative
/
Expression des gènes
/
Régulation positive
Limites du sujet:
Adolescent
/
Adulte
/
Enfant
/
Enfant d'âge préscolaire
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Experimental & Molecular Medicine
Année:
2004
Type:
Article
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