Study on the Activation of Mast Cell by Seminal Plasma in vitro and in vivo I. Effects of Seminal Plasma on the Degranulations and Cutaneous Reactions of Skin Mast Cell in vivo / 체질인류학회지

ABSTRACT

Human seminal plasma (HSP)-induced hypersensitivity IS one of the serious complications associated with sexual intercourse The clinical manifestations of HSP-induced hypersensitivity may be induced by the release of mast cell-mediator by HSP however the effects of HSP on mast cell activity have not been Identified The purpose of this study is to determine If the pathogenesis of seminal plasma (SP)-induced hypeisensitivity IS mediated by mast cells or not To evaluate the reactions of mast cells to SP in VlVO the degranulations of skill mast cells changes of ear thickness, and cutaneous reactions were measured by morphometric method Ammals of normal or positive control groups were treated with phosphate buffered salme (PES) solution or compound 48/80 (2 5 mg/ml m PES) By light microscopy the degranuiations of mice ear mast cells were observed following treatments with HSP, rat semmal plasma (RSP), or compound 48/80 The degranulation rate (DR) of normal group treated with PES solution was 27 8% DR of those with HSP RSP, and compound 48/80 were 70 5% 90 1%, 96 0% respectively The ear swellings were noted m mice treated with HSP RSP, and compound 48/80 The cutaneous reactions m rat skin were noted when HSP or RSP or compound 48/80 were treated These results suggest that SP contains some substances activating skin mast cells and those increasing of vascular permeabilities In mice and I rats skin